Reference
Citation
Casas-Vila, N., Bluhm, A., Sayols, S., Dinges, N., Dejung, M., Altenhein, T., Kappei, D., Altenhein, B., Roignant, J.Y., Butter, F. (2017). The developmental proteome of Drosophila melanogaster.  Genome Res. 27(7): 1273--1285.
FlyBase ID
FBrf0235991
Publication Type
Research paper
Abstract
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila's life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.
PubMed ID
PubMed Central ID
PMC5495078 (PMC) (EuropePMC)
Related Publication(s)
FlyBase analysis
FlyBase incorporation of the Casas-Vila et al., 2017 developmental proteome.
dos Santos and Emmert, 2018, FlyBase incorporation of the Casas-Vila et al., 2017 developmental proteome. [FBrf0238291]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Res.
    Title
    Genome Research
    Publication Year
    1995-
    ISBN/ISSN
    1088-9051
    Data From Reference
    Genes (8)
    Datasets (96)